Thursday, 27th January, 2022, 12:00

New genes and alternative isoforms detected with Oxford Nanopore direct RNA

PRBB Computational Genomics Seminar. Chair: Mar Albà (Head of Evolutionary Genomics Group).

Oxford Nanopore Technologies (ONT) direct RNA (dRNA) sequencing offers several important advantages over previous RNA-Seq approaches: each long sequencing read corresponds to a single mRNA molecule, it is possible to estimate poly(A) tail length, and one can obtain information on RNA modifications. In the present work, we use dRNA to obtain a more comprehensive transcriptome of the model yeast species , S. pombe, which, in contrast to S. cerevisiae, is intron-rich. By using dRNA we uncover many high abundant transcript isoforms, some of which are likely to be translated into proteins. We also observe that lowly expressed transcripts, and a subset of the intron retention isoforms, have abnormally long poly(A) tails, suggesting that poly(A) tail can modulate gene expression. We also use dRNA in combination with Ribo-Seq to identify novel genes in the species.

Zoom webinar: https://us02web.zoom.us/j/81073249721 / Password: 788137

Speaker: José Carlos Montañés, Evolutionary Genomics Group - Research Programme on Biomedical Informatics (GRIB, IMIM/UPF).

Room Online