Thursday, 16th February, 2023, 12:00

Benchmarking of long-read transcriptomics methods; results and insights from the LRGASP project

PRBB Computational Genomics Seminar. Chair: Mar Albà

Long reads sequencing is the Method of the Year 2022 according to Nature Methods. Applied to transcriptome analysis Long reads allow the sequencing of full-length transcripts in one single read and facilities the study of transcript variants.

However the technology is still noisy. The LRGASP project is an initiative to assess the quality of long reads methods for transcript identification and quantification. Long reads data was generated for different organisms, library preparation methods and sequencing platforms (PaBio, Nanopore and Illumina) and launched to the community for transcript models predictions. More than 130 transcriptome predictions datasets were generated by 13 different bioinformatics tools, analyzed with SQANTI and compared. In this seminar I will present the results of the LRGASP Challenges 1 and 3, which deal respectively with the identification of transcripts with and without a good genome annotation. Novel insights about transcriptome diversity will be discussed.

Zoom webinar: https://us02web.zoom.us/j/84212222322?pwd=VmVIdHo3czlFcVZUSDhscnQ0RzN5Zz09

Speaker: Ana Conesa, Institute for Integrative Systems Biology (I2SysBio)

Room Online